JoVE | Peer Reviewed Scientific Video Journal

Biology

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons

Joseph Harris¹, Hyuna Lee¹, Behrad Vahidi¹, Christina Tu², David Cribbs³, Noo Li Jeon¹, Carl Cotman³

¹Department of Biomedical Engineering, University of California, Irvine (UCI), ²Stem Cell Research Center, University of California, Irvine (UCI), ³Institute for Brain Aging and Dementia, University of California, Irvine (UCI)

In this video we demonstrate the technique of soft lithography with polydimethyl siloxane (PDMS) which we use to farbricate a microfluidic device for culturing neurons.

Biology

Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device

Joseph Harris¹, Hyuna Lee¹, Christina Tu Tu², David Cribbs³, Carl Cotman³, Noo Li Jeon¹

¹Department of Biomedical Engineering, University of California, Irvine (UCI), ²Stem Cell Research Center, University of California, Irvine (UCI), ³Institute for Brain Aging and Dementia, University of California, Irvine (UCI)

In this video we demonstrate the preparation of E18 Cortical Rat Neurons.

Biology

Non-plasma Bonding of PDMS for Inexpensive Fabrication of Microfluidic Devices

Joseph Harris¹, Hyuna Lee¹, Behrad Vahidi¹, Cristina Tu², David Cribbs³, Carl Cotman³, Noo Li Jeon¹

¹Department of Biomedical Engineering, University of California, Irvine (UCI), ²Stem Cell Research Center, University of California, Irvine (UCI), ³Institute for Brain Aging and Dementia, University of California, Irvine (UCI)

In this video we demonstrate how to use the neuron microfluidic device without plasma bonding.

Neuroscience

Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons

Tharkika Nagendran^1,2, Valerie Poole³, Joseph Harris³, Anne Marion Taylor^1,2,3

¹UNC Neuroscience Center, ²UNC/NC State Joint Department of Biomedical Engineering, UNC, ³Xona Microfluidics, LLC

This protocol describes the use of plastic chips to culture and compartmentalize primary murine neurons. These chips are preassembled, user-friendly, and compatible with high-resolution, live, and fluorescence imaging. This protocol describes how to plate rat hippocampal neurons within these chips and perform fluidic isolation, axotomy and immunostaining.

Neuroscience

Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips

Smita R. Paranjape^1,2, Tharkika Nagendran^1,2, Valerie Poole³, Joseph Harris³, Anne Marion Taylor^1,2,3

¹UNC Neuroscience Center, ²UNC/NC State Joint Department of Biomedical Engineering, ³Xona Microfluidics, LLC

This protocol demonstrates the use of compartmentalized microfluidic chips, injection molded in a cyclic olefin copolymer to cultured neurons differentiated from human stem cells. These chips are preassembled and easier-to-use than traditional compartmentalized poly(dimethylsiloxane) devices. Multiple common experimental paradigms are described here, including viral labeling, fluidic isolation, axotomy, and immunostaining.

Xona Microfluidics, LLC

5 ARTICLES PUBLISHED IN JoVE

Biology

Biology

Biology

Neuroscience

Neuroscience