Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In VitroKerry Tedford 1, Laura Tech 1, Michael Steiner 1, Mark Korthals 1, Klaus-Dieter Fischer 1
1Institute of Biochemistry and Cell Biology, OVGU University of Magdeburg
Marginal zone B cells (MZBs) respond to the force of shear flow by re-orienting their migration path up the flow. This protocol shows how to record and analyze the migration using a fluidics unit, pump, microscope imaging system, and free software.
