ProDigest

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

Jenni Firrman¹, LinShu Liu¹, Pieter Van den Abbeele², Ceylan Tanes³, Kyle Bittinger³, Peggy Tomasula¹

¹Dairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, ²ProDigest, ³Division of Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Philadelphia

Here, we present a protocol for culturing the gut microbiota of the colon in vitro, using a series of bioreactors that simulate the physiological conditions of the gastro intestinal tract.