Applying Advanced In Vitro Culturing Technology to Study the Human Gut MicrobiotaJenni Firrman 1, LinShu Liu 1, Pieter Van den Abbeele 2, Ceylan Tanes 3, Kyle Bittinger 3, Peggy Tomasula 1
1Dairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 2ProDigest, 3Division of Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Philadelphia
Here, we present a protocol for culturing the gut microbiota of the colon in vitro, using a series of bioreactors that simulate the physiological conditions of the gastro intestinal tract.
