Celetrix LLC
2 ARTICLES PUBLISHED IN JoVE
Environment
Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples
Huan Chen *1,2, Ian Arthur Palmer *1, Jian Chen 1,2, Ming Chang 1,2, Stephen L. Thompson 3, Fengquan Liu 2, Zheng Qing Fu 1
1Department of Biological Sciences, University of South Carolina, 2Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, 3Department of Instructions and Teacher Education, University of South Carolina
Citrus Greening is a particularly destructive disease affecting citrus crops globally. Presented here is a simple method using PCR and genomic DNA extraction of citrus leaf tissue for the accurate and precise identification of the citrus greening pathogen, Candidatus liberibacter spp.
Genetics
CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
Linyuan Ma *1, Lydia Jang *1, Jian Chen 2, Jun Song 1, Dongshan Yang 1, Jifeng Zhang 1, Y. Eugene Chen 1, Jie Xu 1
1Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, 2Celetrix LLC
Presented here is a protocol for efficient CRISPR/Cas9 ribonucleoprotein-mediated gene editing in mammalian cells using tube electroporation.
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