Accedi

Celetrix LLC

2 ARTICLES PUBLISHED IN JoVE

Environment

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

Huan Chen^*1,2, Ian Arthur Palmer^*1, Jian Chen^1,2, Ming Chang^1,2, Stephen L. Thompson³, Fengquan Liu², Zheng Qing Fu¹

¹Department of Biological Sciences, University of South Carolina, ²Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, ³Department of Instructions and Teacher Education, University of South Carolina

Citrus Greening is a particularly destructive disease affecting citrus crops globally. Presented here is a simple method using PCR and genomic DNA extraction of citrus leaf tissue for the accurate and precise identification of the citrus greening pathogen, Candidatus liberibacter spp.

Genetics

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Linyuan Ma^*1, Lydia Jang^*1, Jian Chen², Jun Song¹, Dongshan Yang¹, Jifeng Zhang¹, Y. Eugene Chen¹, Jie Xu¹

¹Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, ²Celetrix LLC

Presented here is a protocol for efficient CRISPR/Cas9 ribonucleoprotein-mediated gene editing in mammalian cells using tube electroporation.