MilliporeSigma
2 ARTICLES PUBLISHED IN JoVE
Biology
Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
Haley R. Pugsley 1
1Amnis Biology Department, MilliporeSigma
Here, multispectral imaging flow cytometry with an analytical feature that compares bright detail images of 3 autophagy markers and quantifies their co-localization, along with LC3 spot counting, was used to measure autophagy in an objective, quantitative, and statistically robust manner.
Measuring Interleukin-6 in Human Serum from Children with a Pediatric Concussion using a Single Molecule Counting Platform
Nicole D. Osier 1,2, David Hutto 3, Claire Slote 1, Adam S. Venable 4
1School of Nursing, University of Texas at Austin, 2Department of Neurology, University of Texas at Austin, 3Cockrell School of Engineering, University of Texas at Austin, 4MilliporeSigma
Methods are described for ultrasensitive quantification of biomarker proteins using an immunoassay system capable of single molecule counting. The platform can be used to quantify a wide array of proteins in a variety of tissues from several species. This exemplar assays interleukin-6 in human serum from pediatric concussion patients.
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