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MilliporeSigma

2 ARTICLES PUBLISHED IN JoVE

Biology

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

¹Amnis Biology Department, MilliporeSigma

Here, multispectral imaging flow cytometry with an analytical feature that compares bright detail images of 3 autophagy markers and quantifies their co-localization, along with LC3 spot counting, was used to measure autophagy in an objective, quantitative, and statistically robust manner.

Measuring Interleukin-6 in Human Serum from Children with a Pediatric Concussion using a Single Molecule Counting Platform

Nicole D. Osier^1,2, David Hutto³, Claire Slote¹, Adam S. Venable⁴

¹School of Nursing, University of Texas at Austin, ²Department of Neurology, University of Texas at Austin, ³Cockrell School of Engineering, University of Texas at Austin, ⁴MilliporeSigma

Methods are described for ultrasensitive quantification of biomarker proteins using an immunoassay system capable of single molecule counting. The platform can be used to quantify a wide array of proteins in a variety of tissues from several species. This exemplar assays interleukin-6 in human serum from pediatric concussion patients.