Washington University school of medicine in Saint Louis

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

Valerie P. O'Brien^1,2,6, Matthew S. Joens^3,7, Amanda L. Lewis^1,2,4,8, Nicole M. Gilbert^2,5

¹Department of Molecular Microbiology, Washington University School of Medicine in Saint Louis, ²Center for Women’s Infectious Disease Research, Washington University School of Medicine in Saint Louis, ³Center for Cellular Imaging, Washington University School of Medicine in Saint Louis, ⁴Department of Obstetrics and Gynecology, Washington University School of Medicine in Saint Louis, ⁵Department of Pediatrics, Washington University School of Medicine in Saint Louis, ⁶Fred Hutchinson Cancer Research Center, ⁷TESCAN USA, Inc., ⁸University of California San Diego

A mouse model of uropathogenic E. coli (UPEC) transurethral inoculation to establish latent intracellular bladder reservoirs and subsequent bladder exposure to G. vaginalis to induce recurrent UPEC UTI is demonstrated. Also demonstrated are the enumeration of bacteria, urine cytology, and in situ bladder fixation and processing for scanning electron microscopy.

Immunology and Infection

Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

Sydney R. Morrill^1,2,3, Kavita Agarwal^2,3, Sudeshna Saha^2,3, Warren G. Lewis^2,3, Nicole M. Gilbert⁴, Amanda L. Lewis^2,3

¹Division of Biology and Biomedical Sciences, Washington University School of Medicine, ²Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, ³Glycobiology Research and Training Center, UCSD, ⁴Department of Pediatrics, Washington University School of Medicine

The protocol presents a mouse model of vaginal colonization with anaerobically cultured human vaginal bacteria. We focus on Gardnerella vaginalis, while including suggestions for Prevotella bivia and Fusobacterium nucleatum. This protocol can also be used as a guide for vaginal inoculations and viable recovery of other anaerobically grown bacteria.