Tampere University

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Hanna Luukinen¹, Milka Marjut Hammarén¹, Leena-Maija Vanha-aho¹, Mataleena Parikka^1,2

¹Faculty of Medicine and Life Sciences, University of Tampere, ²Oral and Maxillofacial Unit, Tampere University Hospital

Here, we present a protocol to model human tuberculosis in an adult zebrafish using its natural pathogen Mycobacterium marinum. Extracted DNA and RNA from the internal organs of infected zebrafish can be used to reveal the total mycobacterial loads in the fish and the host's immune responses with qPCR.

Medicine

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos

Ashok Aspatwar¹, Milka Marjut Hammaren¹, Mataleena Parikka^1,2, Seppo Parkkila^1,3

¹Faculty of Medicine and Health Technology, Tampere University, ²Oral and Maxillofacial Unit, Tampere University Hospital, ³Fimlab Ltd, Tampere University Hospital

Zebrafish embryos are used for evaluating the toxicity of chemical compounds. They develop externally and are sensitive to chemicals, allowing detection of subtle phenotypic changes. The experiment only requires a small amount of compound, which is directly added to the plate containing embryos, making the testing system efficient and cost-effective.

JoVE Core

A Modeling and Simulation Method for Preliminary Design of an Electro-Variable Displacement Pump

Xu Han¹, Peng Zhang², Tatiana Minav³, Yongling Fu¹, Jian Fu¹

¹School of Mechanical Engineering and Automation, Beihang University, ²Beijing Institute of Precision Mechatronics and Controls, ³Innovative Hydraulics and Automation, Tampere University

A simulation model specifically supporting the preliminary design of an electro-variable displacement pump (EVDP) is developed and partially verified by experiments. The control performance, life, reliability, etc., can all be evaluated using the proposed model, which covers the main performance requirements under the EVDP preliminary design task.