Ashoka University

1 ARTICLES PUBLISHED IN JoVE

Biology

Enhanced Extraction of Low-Molecular Weight DNA from Wastewater for Comprehensive Assessment of Antimicrobial Resistance

Jyotsna Karan¹, Sreya Mandal¹, Gulafsha Khan¹, Harshit Arya¹, Laasya Samhita¹

¹Ashoka University

Here, we present a simple technique to assess environmental antimicrobial resistance (AMR) by enhancing the proportion of low-molecular-weight extracellular DNA. Prior treatment with 20%-30% PEG and 1.2 M NaCl allows detection of both genomic and horizontally transferred AMR genes. The protocol lends itself to a kit-free process with additional optimization.