Max Planck Institute of Neurobiology
2 ARTICLES PUBLISHED IN JoVE
Behavior
Two-photon Calcium Imaging in Mice Navigating a Virtual Reality Environment
Marcus Leinweber *1,2, Pawel Zmarz *1,2, Peter Buchmann 3, Paul Argast 1, Mark Hübener 2, Tobias Bonhoeffer 2, Georg B. Keller 1,2
1Friedrich Miescher Institute for Biomedical Research, 2Max Planck Institute of Neurobiology, 3Department of Biosystems Science and Engineering, ETH Zurich
Here we describe the experimental procedures involved in two-photon imaging of mouse cortex during behavior in a virtual reality environment.
Neuroscience
Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
Simone Wanderoy *1,2, Alina Rühmkorf *1,2, Angelika B. Harbauer 2,3,4
1TUM Medical Graduate Center, Technical University of Munich, 2Max Planck Institute of Neurobiology, 3TUM School of Medicine, Institute of Neuronal Cell Biology, Technical University of Munich, 4Munich Cluster for Systems Neurology
The present protocol describes the seeding and staining of neuronal mitochondria in microfluidic chambers. The fluidic pressure gradient in these chambers allows for the selective treatment of mitochondria in axons to analyze their properties in response to pharmacological challenges without affecting the cell body compartment.
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