Accedi

Max Planck Institute of Neurobiology

2 ARTICLES PUBLISHED IN JoVE

Behavior

Two-photon Calcium Imaging in Mice Navigating a Virtual Reality Environment

Marcus Leinweber^*1,2, Pawel Zmarz^*1,2, Peter Buchmann³, Paul Argast¹, Mark Hübener², Tobias Bonhoeffer², Georg B. Keller^1,2

¹Friedrich Miescher Institute for Biomedical Research, ²Max Planck Institute of Neurobiology, ³Department of Biosystems Science and Engineering, ETH Zurich

Here we describe the experimental procedures involved in two-photon imaging of mouse cortex during behavior in a virtual reality environment.

Neuroscience

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

Simone Wanderoy^*1,2, Alina Rühmkorf^*1,2, Angelika B. Harbauer^2,3,4

¹TUM Medical Graduate Center, Technical University of Munich, ²Max Planck Institute of Neurobiology, ³TUM School of Medicine, Institute of Neuronal Cell Biology, Technical University of Munich, ⁴Munich Cluster for Systems Neurology

The present protocol describes the seeding and staining of neuronal mitochondria in microfluidic chambers. The fluidic pressure gradient in these chambers allows for the selective treatment of mitochondria in axons to analyze their properties in response to pharmacological challenges without affecting the cell body compartment.