Saarland University Medical Center

3 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Henrike Julich-Haertel¹, Marina Tiwari¹, Christina Mehrfeld¹, Elmar Krause², Miroslaw Kornek¹, Veronika Lukacs-Kornek¹

¹Department of Medicine II, Saarland University Medical Center, ²Department of Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), University of Saarland

Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.

Medicine

Electrochemotherapy in 3D Ocular Melanoma Spheroids using a Customized Electrode

Miltiadis Fiorentzis^1,2, Arne Viestenz¹, Berthold Seitz³, Sarah E Coupland⁴, Joana Heinzelmann¹

¹Department of Ophthalmology, University Hospital Halle (Saale), Martin-Luther University Halle-Wittenberg, ²Department of Ophthalmology, University Hospital Essen, Duisburg Essen University, ³Department of Ophthalmology, Saarland University Medical Center, ⁴Liverpool Ocular Oncology Research Group, Department of Molecular and Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool

Here, we present a protocol for the development of 3D conjunctival and uveal melanoma spheroids and the use of hand-held customized electrodes for in vitro electrochemotherapy of 3D spheroids in a culture well. This offers new perspectives in the use of electrochemotherapy in ocular melanoma treatment.

Medicine

Diagnosis and Surgical Treatment of Human Brucellar Spondylodiscitis

Yakefu Abulizi^*1, Xiaoyu Cai^*1,2,3, Tao Xu¹, Chuanhui Xun¹, Weibin Sheng¹, Liang Gao^2,3, Maierdan Maimaiti¹

¹Department of Spine Surgery, First Affiliated Hospital of Xinjiang Medical University, ²Center of Experimental Orthopaedics, Saarland University Medical Center, ³Sino Euro Orthopaedics Network (SEON)

We describe a clinical algorithm, based on decades of front-line experience of diagnosis and surgical treatment of human Brucellar spondylodiscitis in the largest medical center of the China's Xinjiang Pastoral Area.