University of Artois

2 ARTICLES PUBLISHED IN JoVE

Biology

A High Output Method to Isolate Cerebral Pericytes from Mouse

Anupriya Mehra¹, Lucie Dehouck¹, Elodie Vandenhaute¹, Marc Fatar², Laurence Fenart¹, Fabien Gosselet¹

¹Laboratory of the Blood Brain Barrier, University of Artois, ²Department of Neurology, Universitätsmedizin Mannheim, Heidelberg University

We present a protocol for the extraction of murine cerebral pericytes. Based on an antibiotic-free enrichment oriented pericyte extraction, this protocol is a valuable tool for in vitro studies providing high purity and high yield, thus decreasing the number of experimental animals used.

Biology

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

Eleonora Rizzi^*1, Clémence Deligne^*1, Lucie Dehouck¹, Roberta Bilardo², Yasutero Sano³, Fumitaka Shimizu³, Takashi Kanda³, Marina Resmini², Fabien Gosselet¹, Marie-Pierre Dehouck¹, Caroline Mysiorek¹

¹Univ. Artois, UR 2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), ²Queen Mary University of London, Department of Chemistry, ³Yamaguchi University Graduate School of Medicine, Department of Neurology and Clinical Neuroscience

This protocol describes a method to establish a human blood-brain barrier (BBB) in vitro model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport experiments.