University of Artois
2 ARTICLES PUBLISHED IN JoVE
Biology
A High Output Method to Isolate Cerebral Pericytes from Mouse
Anupriya Mehra 1, Lucie Dehouck 1, Elodie Vandenhaute 1, Marc Fatar 2, Laurence Fenart 1, Fabien Gosselet 1
1Laboratory of the Blood Brain Barrier, University of Artois, 2Department of Neurology, Universitätsmedizin Mannheim, Heidelberg University
We present a protocol for the extraction of murine cerebral pericytes. Based on an antibiotic-free enrichment oriented pericyte extraction, this protocol is a valuable tool for in vitro studies providing high purity and high yield, thus decreasing the number of experimental animals used.
Biology
A Triple Culture Cell System Modeling the Human Blood-Brain Barrier
Eleonora Rizzi *1, Clémence Deligne *1, Lucie Dehouck 1, Roberta Bilardo 2, Yasutero Sano 3, Fumitaka Shimizu 3, Takashi Kanda 3, Marina Resmini 2, Fabien Gosselet 1, Marie-Pierre Dehouck 1, Caroline Mysiorek 1
1Univ. Artois, UR 2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), 2Queen Mary University of London, Department of Chemistry, 3Yamaguchi University Graduate School of Medicine, Department of Neurology and Clinical Neuroscience
This protocol describes a method to establish a human blood-brain barrier (BBB) in vitro model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport experiments.
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