University of Southern California, Keck School of Medicine
2 ARTICLES PUBLISHED IN JoVE
Biology
Mouse Adrenal Chromaffin Cell Isolation
Aaron Kolski-Andreaco 1, Haijiang Cai 2,3, D. Spencer Currle 4, K. George Chandy 1, Robert H. Chow 2,3
1Department of Physiology and Biophysics, University of California, Irvine (UCI), 2Department of Physiology and Biophysics, University of Southern California, Keck School of Medicine, 3Zilkha Neurogenetic Institute, University of Southern California, Keck School of Medicine, 4Department of Developmental and Cell Biology, University of California, Irvine (UCI)
Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting. This protocol illustrates the method used to dissect the adrenals and then isolate the medullary region by stripping away the adrenal cortex. The digestion of the medulla into single chromaffin cells is then demonstrated.
Medicine
Point of Care Transcranial Color-Coded Duplex Ultrasound of the Middle Cerebral Artery
Fawaz Philip Tarzi 1, Sebina Bulic 1, Stacey Ho 1, Pravesh Saini 1, Andrei Alexandrov 2, Yuriy S. Bronshteyn 3
1Department of Neurology, University of Southern California, Keck school of Medicine, 2Department of Neurology, University of Arizona, College of Medicine, 3Department of Anesthesiology, Duke University School of Medicine
Transcranial ultrasound is an essential tool for monitoring patients with various neurological conditions. Although it is commonly used in a protocolized fashion in consultative studies, the brain has been overlooked in many protocols utilizing point-of-care ultrasound (PoCUS). This study proposes a PoCUS image acquisition protocol.
