University of California Santa Cruz - UCSC
3 ARTICLES PUBLISHED IN JoVE
Biology
Bacterial Gene Expression Analysis Using Microarrays
Sinem Beyhan 1, Fitnat Yildiz 1
1Department of Environmental Toxicology, University of California Santa Cruz - UCSC
Bacterial Gene Expression Analysis Using Microarrays
Biology
Vibrio cholerae: Model Organism to Study Bacterial Pathogenesis - Interview
Fitnat Yildiz 1
1Department of Environmental Toxicology, University of California Santa Cruz - UCSC
Vibrio cholerae: Model Organism to Study Bacterial Pathogenesis - Interview
Biology
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
Chris Troll 1, David Alexander 1, Jennifer Allen 1, Jacob Marquette 1, Manel Camps 1
1Department of Microbiology & Environmental Toxicology, University of California Santa Cruz - UCSC
Here we demonstrate a simple protocol to create a random mutant library for a given target sequence. We show how this method, which is performed in vivo in Escherichia coli, can be coupled with functional selections to evolve new enzymatic activities.
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