Sigma Aldrich
2 ARTICLES PUBLISHED IN JoVE
JoVE Journal
Sigma's Non-specific Protease Activity Assay - Casein as a Substrate
Carrie Cupp-Enyard 1
1Sigma Aldrich
Proteases break peptide bonds. In the lab, it is often necessary to measure and/or compare the activity of proteases. Sigma's non-specific protease activity assay may be used as a standardized procedure to determine the activity of proteases.
Biology
Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
Carrie Cupp-Enyard 1
1Sigma Aldrich
The Protease Fluorescent Detection Kit is designed for the measurement of protease activity using fluorometry. It is also suitable for detection of trace amounts of protease contamination. The method is based on the proteolytic hydroysis of a proprietary formulation of a FITC-labeled casein substrate.
