Sigma Aldrich

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Sigma's Non-specific Protease Activity Assay - Casein as a Substrate

Proteases break peptide bonds. In the lab, it is often necessary to measure and/or compare the activity of proteases. Sigma's non-specific protease activity assay may be used as a standardized procedure to determine the activity of proteases.

Biology

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

Carrie Cupp-Enyard¹

¹Sigma Aldrich

The Protease Fluorescent Detection Kit is designed for the measurement of protease activity using fluorometry. It is also suitable for detection of trace amounts of protease contamination. The method is based on the proteolytic hydroysis of a proprietary formulation of a FITC-labeled casein substrate.