City of Hope Cancer Center
2 ARTICLES PUBLISHED IN JoVE
Biology
Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs
Linxia Zhang 1, Christina Chan 1
1Department of Chemical Engineering and Materials Science, City of Hope Cancer Center
Rat MSCs were isolated from femurs and tibias and then enriched by magnetic cell sorting. Sorted cells were confirmed for the expression of surface markers by flow cytometry. These cells were also cultured at clonal density to form single colonies and then these colonies were separated by cloning cylinders.
Neuroscience
An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons
Chun Liu 1, Christina Chan 1,2
1Department of Chemical Engineering and Materials Science, Michigan State University, 2Department of Biochemistry and Molecular Biology, Michigan State University
This protocol describes the isolation of dorsal root ganglion (DRG) neurons isolated from rats and the culture of DRG neurons on a static pre-stretched cell culture system to enhance axon alignment, with subsequent co-culture of Schwann Cells (SCs) to promote myelination.
