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City of Hope Cancer Center

2 ARTICLES PUBLISHED IN JoVE

Biology

Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs

¹Department of Chemical Engineering and Materials Science, City of Hope Cancer Center

Rat MSCs were isolated from femurs and tibias and then enriched by magnetic cell sorting. Sorted cells were confirmed for the expression of surface markers by flow cytometry. These cells were also cultured at clonal density to form single colonies and then these colonies were separated by cloning cylinders.

Neuroscience

An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons

Chun Liu¹, Christina Chan^1,2

¹Department of Chemical Engineering and Materials Science, Michigan State University, ²Department of Biochemistry and Molecular Biology, Michigan State University

This protocol describes the isolation of dorsal root ganglion (DRG) neurons isolated from rats and the culture of DRG neurons on a static pre-stretched cell culture system to enhance axon alignment, with subsequent co-culture of Schwann Cells (SCs) to promote myelination.