University Health Network (UHN)
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
Christina Farr 1, Stuart Berger 1,2
1Immunology, University of Toronto, 2Arthritis and Immune Disorder Research Centre, University Health Network (UHN)
This article will detail the protocol for measuring calpain activity in fixed and living cells using flow cytometry.
Immunology and Infection
Isolation and Identification of Extravascular Immune Cells of the Heart
Laura Aronoff 1,2, Slava Epelman 1,2,3,4,5, Xavier Clemente-Casares 1,2
1Toronto General Hospital Research Institute, University Health Network (UHN), 2Dept of Laboratory Medicine and Pathobiology, University of Toronto, 3Dept of Immunology, University of Toronto, 4Peter Munk Cardiac Centre, 5Ted Rogers Centre for Heart Research
This protocol presents a simple and efficient method to isolate, identify and quantify immune cells residing in the myocardium of mice during steady state or inflammation. The protocol combines enzymatic and mechanical digestion for the generation of a single cell suspension that can be further analyzed by flow cytometry.
关于 JoVE
版权所属 © 2024 MyJoVE 公司版权所有，本公司不涉及任何医疗业务和医疗服务。