University Health Network (UHN)

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

Christina Farr¹, Stuart Berger^1,2

¹Immunology, University of Toronto, ²Arthritis and Immune Disorder Research Centre, University Health Network (UHN)

This article will detail the protocol for measuring calpain activity in fixed and living cells using flow cytometry.

Immunology and Infection

Isolation and Identification of Extravascular Immune Cells of the Heart

Laura Aronoff^1,2, Slava Epelman^1,2,3,4,5, Xavier Clemente-Casares^1,2

¹Toronto General Hospital Research Institute, University Health Network (UHN), ²Dept of Laboratory Medicine and Pathobiology, University of Toronto, ³Dept of Immunology, University of Toronto, ⁴Peter Munk Cardiac Centre, ⁵Ted Rogers Centre for Heart Research

This protocol presents a simple and efficient method to isolate, identify and quantify immune cells residing in the myocardium of mice during steady state or inflammation. The protocol combines enzymatic and mechanical digestion for the generation of a single cell suspension that can be further analyzed by flow cytometry.