University of Illinois Chicago - UIC
2 ARTICLES PUBLISHED IN JoVE
Biology
Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
Michael L. Beshiri 1, Abul Islam 2, Dannielle C. DeWaal 1, William F. Richter 1, Jennifer Love 3, Nuria Lopez-Bigas 2, Elizaveta V. Benevolenskaya 1
1Department of Biochemistry and Molecular Genetics, University of Illinois Chicago - UIC, 2Research Unit on Biomedical Informatics, Universitat Pompeu Fabra, 3Genome Technology Core, Whitehead Institute for Biomedical Research
Here we are presenting a chromatin immunoprecipitation (ChIP) procedure for genome-wide location analysis of protein isoforms that differ in a histone-binding domain. We are applying it to ChIP-Seq analysis to identify the targets of the KDM5A/JARID1A/RBP2 histone demethylase.
Biology
Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
Forum H. Bhatt 1, Constance J. Jeffery 1
1Department of Biological Sciences, University of Illinois Chicago - UIC
In this protocol we demonstrate the expression, solubilization, and purification of a recombinantly expressed membrane protein, MexB, as a soluble protein detergent complex. MexB is a multidrug resistance membrane transporter from the opportunistic bacterial pathogen Pseudomonas aeruginosa.
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