Accedi

University of Illinois Chicago - UIC

2 ARTICLES PUBLISHED IN JoVE

Biology

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Michael L. Beshiri¹, Abul Islam², Dannielle C. DeWaal¹, William F. Richter¹, Jennifer Love³, Nuria Lopez-Bigas², Elizaveta V. Benevolenskaya¹

¹Department of Biochemistry and Molecular Genetics, University of Illinois Chicago - UIC, ²Research Unit on Biomedical Informatics, Universitat Pompeu Fabra, ³Genome Technology Core, Whitehead Institute for Biomedical Research

Here we are presenting a chromatin immunoprecipitation (ChIP) procedure for genome-wide location analysis of protein isoforms that differ in a histone-binding domain. We are applying it to ChIP-Seq analysis to identify the targets of the KDM5A/JARID1A/RBP2 histone demethylase.

Biology

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein

Forum H. Bhatt¹, Constance J. Jeffery¹

¹Department of Biological Sciences, University of Illinois Chicago - UIC

In this protocol we demonstrate the expression, solubilization, and purification of a recombinantly expressed membrane protein, MexB, as a soluble protein detergent complex. MexB is a multidrug resistance membrane transporter from the opportunistic bacterial pathogen Pseudomonas aeruginosa.