Japan Science and Technology Corporation (JST)
2 ARTICLES PUBLISHED IN JoVE
Biology
The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System
Masanori Takahashi 1, Noriko Osumi 1,2
1Division of Developmental Neuroscience, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, 2The Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST)
Whole embryo culture technique allows us to culture mouse and rat embryos ex vivo condition during limited periods corresponding to midgestation stages. In this video protocol, we demonstrate our standard procedures of rat whole embryo culture after E12.5 using the rotator-type bottle culture system.
Biology
Preparation of Rat Serum Suitable for Mammalian Whole Embryo Culture
Masanori Takahashi 1,2, Sayaka Makino 3, Takako Kikkawa 3, Noriko Osumi 3
1Graduate School of Medicine, Jichi Medical University, 2Division of Biology, Center for Molecular Medicine, Jichi Medical University, 3Department of Developmental Neuroscience, United Center for Advanced Research and Translational Medicine (ART), Tohoku University School of Medicine
Mammalian whole embryo culture (WEC) is widely used in teratology and developmental biology. Immediately centrifuged rat serum is commonly provided as a medium for both mouse and rat WEC. In this video, we demonstrate our standard protocol for the preparation of high-quality rat serum suitable for mammalian WEC.
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