Accedi

Japan Science and Technology Corporation (JST)

2 ARTICLES PUBLISHED IN JoVE

Biology

The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System

¹Division of Developmental Neuroscience, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, ²The Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST)

Whole embryo culture technique allows us to culture mouse and rat embryos ex vivo condition during limited periods corresponding to midgestation stages. In this video protocol, we demonstrate our standard procedures of rat whole embryo culture after E12.5 using the rotator-type bottle culture system.

Biology

Preparation of Rat Serum Suitable for Mammalian Whole Embryo Culture

Masanori Takahashi^1,2, Sayaka Makino³, Takako Kikkawa³, Noriko Osumi³

¹Graduate School of Medicine, Jichi Medical University, ²Division of Biology, Center for Molecular Medicine, Jichi Medical University, ³Department of Developmental Neuroscience, United Center for Advanced Research and Translational Medicine (ART), Tohoku University School of Medicine

Mammalian whole embryo culture (WEC) is widely used in teratology and developmental biology. Immediately centrifuged rat serum is commonly provided as a medium for both mouse and rat WEC. In this video, we demonstrate our standard protocol for the preparation of high-quality rat serum suitable for mammalian WEC.