John Curtin School of Medical Research, Australian National University
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation
Benjamin J. C. Quah 1, Christopher R. Parish 1
1Department of Immunology, John Curtin School of Medical Research, Australian National University
CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. As such, when a CFSE-labeled cell divides, its progeny have half the amount of fluorescence, which can thereby be used to assess cell division. This article describes the procedures typically used for labeling mouse lymphocytes with CFSE.
Immunology and Infection
The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
Benjamin J. C. Quah 1, Danushka K. Wijesundara 1, Charani Ranasinghe 1, Christopher R. Parish 1
1Department of Immunology, John Curtin School of Medical Research, Australian National University
The ability to monitor T cell responses in detail in vivo is important for the development of our understanding of the immune response. Here we describe the use of fluorescent target arrays (FTAs) in an in vivo T cell assay that assesses >250 parameters simultaneously by flow cytometry.
