John Curtin School of Medical Research, Australian National University

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation

Benjamin J. C. Quah¹, Christopher R. Parish¹

¹Department of Immunology, John Curtin School of Medical Research, Australian National University

CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. As such, when a CFSE-labeled cell divides, its progeny have half the amount of fluorescence, which can thereby be used to assess cell division. This article describes the procedures typically used for labeling mouse lymphocytes with CFSE.

Immunology and Infection

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

Benjamin J. C. Quah¹, Danushka K. Wijesundara¹, Charani Ranasinghe¹, Christopher R. Parish¹

¹Department of Immunology, John Curtin School of Medical Research, Australian National University

The ability to monitor T cell responses in detail in vivo is important for the development of our understanding of the immune response. Here we describe the use of fluorescent target arrays (FTAs) in an in vivo T cell assay that assesses >250 parameters simultaneously by flow cytometry.