University Medical Center Groningen, University of Groningen

4 ARTICLES PUBLISHED IN JoVE

Biology

Isolation of Mouse Salivary Gland Stem Cells

Sarah Pringle^1,2, Lalitha S. Y. Nanduri¹, van der Zwaag Marianne¹, van Os Ronald^1,2, Rob P. Coppes^1,2

¹Department of Cell Biology, University Medical Center Groningen, University of Groningen, ²Department of Radiation Oncology, University Medical Center Groningen, University of Groningen

An optimized protocol for the isolation of stem cells from the mouse salivary gland is described. The method employs enzymatic and mechanical digestion, and permits isolation of salispheres containing cells with characteristics of stem cells.

Medicine

Ex Situ Normothermic Machine Perfusion of Donor Livers

Negin Karimian^1,2, Alix P.M. Matton^1,2, Andrie C. Westerkamp^1,2, Laura C. Burlage^1,2, Sanna op den Dries^1,2, Henri G.D. Leuvenink², Ton Lisman^1,2, Korkut Uygun³, James F. Markmann⁴, Robert J. Porte^1,2

¹Section of Hepato-Pancreato-Biliary Surgery and Liver Transplantation, University of Groningen, University Medical Center Groningen, ²Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, ³Center of Engineering in Medicine/Surgical Services, Massachusetts General Hospital, Harvard Medical School, and Shriners Burns Hospital, ⁴Division of Transplantation, Department of Surgery, Massachusetts General Hospital, Harvard Medical School

Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.

Medicine

Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion

Bote G. Bruinsma^*1, James H. Avruch^*2, Pepijn D. Weeder¹, Gautham V. Sridharan¹, Basak E. Uygun¹, Negin G. Karimian¹, Robert J. Porte³, James F. Markmann², Heidi Yeh², Korkut Uygun¹

¹Center for Engineering in Medicine, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School, ²Transplant Center, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School, ³Section of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University Medical Center Groningen, University of Groningen

We describe a method of ex vivo machine perfusion of human liver grafts at subnormothermic temperature (21 °C).

Medicine

Shunt Surgery, Right Heart Catheterization, and Vascular Morphometry in a Rat Model for Flow-induced Pulmonary Arterial Hypertension

Diederik E. van der Feen¹, Michel Weij², Annemieke Smit-van Oosten², Lysanne M. Jorna¹, Quint A.J. Hagdorn¹, Beatrijs Bartelds¹, Rolf M.F. Berger¹

¹Center for Congenital Heart Diseases, Department of Pediatric Cardiology, Beatrix Children's Hospital, University Medical Center Groningen, University of Groningen, ²Research and Development Facility, University Medical Center Groningen, University of Groningen

This protocol describes a surgical procedure to create a model for flow-induced pulmonary arterial hypertension (PAH) in rats and the procedures to analyze the principle hemodynamic and histological end-points in this model.