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University Medical Center Groningen, University of Groningen

4 ARTICLES PUBLISHED IN JoVE

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Biology

Isolation of Mouse Salivary Gland Stem Cells
Sarah Pringle 1,2, Lalitha S. Y. Nanduri 1, van der Zwaag Marianne 1, van Os Ronald 1,2, Rob P. Coppes 1,2
1Department of Cell Biology, University Medical Center Groningen, University of Groningen, 2Department of Radiation Oncology, University Medical Center Groningen, University of Groningen

An optimized protocol for the isolation of stem cells from the mouse salivary gland is described. The method employs enzymatic and mechanical digestion, and permits isolation of salispheres containing cells with characteristics of stem cells.

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Medicine

Ex Situ Normothermic Machine Perfusion of Donor Livers
Negin Karimian 1,2, Alix P.M. Matton 1,2, Andrie C. Westerkamp 1,2, Laura C. Burlage 1,2, Sanna op den Dries 1,2, Henri G.D. Leuvenink 2, Ton Lisman 1,2, Korkut Uygun 3, James F. Markmann 4, Robert J. Porte 1,2
1Section of Hepato-Pancreato-Biliary Surgery and Liver Transplantation, University of Groningen, University Medical Center Groningen, 2Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, 3Center of Engineering in Medicine/Surgical Services, Massachusetts General Hospital, Harvard Medical School, and Shriners Burns Hospital, 4Division of Transplantation, Department of Surgery, Massachusetts General Hospital, Harvard Medical School

Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.

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Medicine

Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion
Bote G. Bruinsma *1, James H. Avruch *2, Pepijn D. Weeder 1, Gautham V. Sridharan 1, Basak E. Uygun 1, Negin G. Karimian 1, Robert J. Porte 3, James F. Markmann 2, Heidi Yeh 2, Korkut Uygun 1
1Center for Engineering in Medicine, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School, 2Transplant Center, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School, 3Section of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University Medical Center Groningen, University of Groningen

We describe a method of ex vivo machine perfusion of human liver grafts at subnormothermic temperature (21 °C).

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Medicine

Shunt Surgery, Right Heart Catheterization, and Vascular Morphometry in a Rat Model for Flow-induced Pulmonary Arterial Hypertension
Diederik E. van der Feen 1, Michel Weij 2, Annemieke Smit-van Oosten 2, Lysanne M. Jorna 1, Quint A.J. Hagdorn 1, Beatrijs Bartelds 1, Rolf M.F. Berger 1
1Center for Congenital Heart Diseases, Department of Pediatric Cardiology, Beatrix Children's Hospital, University Medical Center Groningen, University of Groningen, 2Research and Development Facility, University Medical Center Groningen, University of Groningen

This protocol describes a surgical procedure to create a model for flow-induced pulmonary arterial hypertension (PAH) in rats and the procedures to analyze the principle hemodynamic and histological end-points in this model.

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