University of North Carolina-Chapel Hill
2 ARTICLES PUBLISHED IN JoVE
Medicine
In Vivo Canine Muscle Function Assay
Martin K. Childers 1, Robert W. Grange 2, Joe N. Kornegay 3
1Department of Neurology and Wake Forest Institute for Regenerative Medicine, Wake Forest University, 2Department of Human Nutrition, Foods and Exercise, Virginia Polytechnic Institute and State University, 3Departments of Pathology and Laboratory Medicine and Neurology and the Gene Therapy Center , University of North Carolina-Chapel Hill
We describe a minimally-invasive and painless method to measure canine hindlimb muscle strength and muscle response to repeated eccentric contractions.
Immunology and Infection
Generation of Organotypic Raft Cultures from Primary Human Keratinocytes
Daniel Anacker 1, Cary Moody 1,2
1Department of Microbiology & Immunology, University of North Carolina-Chapel Hill, 2Lineberger Cancer Center, University of North Carolina-Chapel Hill
An in vitro method to mimic in vivo epithelial differentiation is described. Many viruses target epithelial cells as part of their viral life cycle, and this method provides a means of examining virus:host interactions that more closely resembles that which occurs in vivo. This technique can be used with primary keratinocytes, established cell lines, as well as normal or diseased biopsy tissue.
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