Accedi

University of North Carolina-Chapel Hill

2 ARTICLES PUBLISHED IN JoVE

Medicine

Martin K. Childers¹, Robert W. Grange², Joe N. Kornegay³

¹Department of Neurology and Wake Forest Institute for Regenerative Medicine, Wake Forest University, ²Department of Human Nutrition, Foods and Exercise, Virginia Polytechnic Institute and State University, ³Departments of Pathology and Laboratory Medicine and Neurology and the Gene Therapy Center , University of North Carolina-Chapel Hill

We describe a minimally-invasive and painless method to measure canine hindlimb muscle strength and muscle response to repeated eccentric contractions.

Immunology and Infection

Generation of Organotypic Raft Cultures from Primary Human Keratinocytes

Daniel Anacker¹, Cary Moody^1,2

¹Department of Microbiology & Immunology, University of North Carolina-Chapel Hill, ²Lineberger Cancer Center, University of North Carolina-Chapel Hill

An in vitro method to mimic in vivo epithelial differentiation is described. Many viruses target epithelial cells as part of their viral life cycle, and this method provides a means of examining virus:host interactions that more closely resembles that which occurs in vivo. This technique can be used with primary keratinocytes, established cell lines, as well as normal or diseased biopsy tissue.