University of Pennsylvania School of Medicine
2 ARTICLES PUBLISHED IN JoVE
Biology
High-throughput Yeast Plasmid Overexpression Screen
Michael S. Fleming 1, Aaron D. Gitler 2
1Neuroscience Graduate Group, University of Pennsylvania School of Medicine , 2Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine
Here we describe a plasmid overexpression screen in Saccharomyces cerevisiae, using an arrayed plasmid library and a high-throughput yeast transformation protocol with a liquid handling robot.
Identification of Cells with Markers of Cellular Senescence in Human Formalin-fixed, Paraffin-embedded Brain Tissue Sections
Elizabeth P. Crowe *1, Alessandro Bitto *1, Rekha Bhat 1, Frederick Bradley Johnson 2, John Q. Trojanowski 2, Christian Sell 1, Claudio Torres 1
1Department of Pathology and Laboratory Medicine, Drexel University College of Medicine, 2Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine
We describe an immunofluorescence procedure to detect markers of cellular senescence in human formalin-fixed, paraffin-embedded brain sections. In contrast to immunohistochemistry, immunofluorescence permits semi-quantitative measurements and multiple staining on the same slide, which allows detecting different cell types more rigorously and assessing the abundance of senescence markers more objectively.
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