University of Pennsylvania School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Biology

High-throughput Yeast Plasmid Overexpression Screen

¹Neuroscience Graduate Group, University of Pennsylvania School of Medicine , ²Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine

Here we describe a plasmid overexpression screen in Saccharomyces cerevisiae, using an arrayed plasmid library and a high-throughput yeast transformation protocol with a liquid handling robot.

Identification of Cells with Markers of Cellular Senescence in Human Formalin-fixed, Paraffin-embedded Brain Tissue Sections

Elizabeth P. Crowe^*1, Alessandro Bitto^*1, Rekha Bhat¹, Frederick Bradley Johnson², John Q. Trojanowski², Christian Sell¹, Claudio Torres¹

¹Department of Pathology and Laboratory Medicine, Drexel University College of Medicine, ²Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine

We describe an immunofluorescence procedure to detect markers of cellular senescence in human formalin-fixed, paraffin-embedded brain sections. In contrast to immunohistochemistry, immunofluorescence permits semi-quantitative measurements and multiple staining on the same slide, which allows detecting different cell types more rigorously and assessing the abundance of senescence markers more objectively.