Ottawa Hospital Research Institute (OHRI)
2 ARTICLES PUBLISHED IN JoVE
Medicine
Ex Vivo Infection of Live Tissue with Oncolytic Viruses
Jean-Simon Diallo 1, Dominic Roy 1, Hesham Abdelbary 1, Naomi De Silva 1, John C. Bell 1
1Center for Innovative Cancer Research, Ottawa Hospital Research Institute (OHRI)
Oncolytic viruses are promising for cancer therapeutics. The ability to ascertain the infectability of live tissue specimens obtained from patients prior to treatment is a unique advantage of this therapeutic approach. This protocol describes how to process tissues for ex vivo infection with oncolytic virus and subsequent viral quantification.
Biology
High-throughput Titration of Luciferase-expressing Recombinant Viruses
Vanessa Garcia 1,2, Ramya Krishnan 1,2, Colin Davis 1,2, Cory Batenchuk 1,2, Fabrice Le Boeuf 1,3, Hesham Abdelbary 1,3, Jean-Simon Diallo 1,2
1Center for Innovative Cancer Research, Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 3Faculty of Medicine, University of Ottawa
This article presents a high-throughput luciferase expression-based method of titrating various RNA and DNA viruses using automated and manual liquid handlers.
