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Ottawa Hospital Research Institute (OHRI)

2 ARTICLES PUBLISHED IN JoVE

Medicine

Ex Vivo Infection of Live Tissue with Oncolytic Viruses

Jean-Simon Diallo¹, Dominic Roy¹, Hesham Abdelbary¹, Naomi De Silva¹, John C. Bell¹

¹Center for Innovative Cancer Research, Ottawa Hospital Research Institute (OHRI)

Oncolytic viruses are promising for cancer therapeutics. The ability to ascertain the infectability of live tissue specimens obtained from patients prior to treatment is a unique advantage of this therapeutic approach. This protocol describes how to process tissues for ex vivo infection with oncolytic virus and subsequent viral quantification.

Biology

High-throughput Titration of Luciferase-expressing Recombinant Viruses

Vanessa Garcia^1,2, Ramya Krishnan^1,2, Colin Davis^1,2, Cory Batenchuk^1,2, Fabrice Le Boeuf^1,3, Hesham Abdelbary^1,3, Jean-Simon Diallo^1,2

¹Center for Innovative Cancer Research, Ottawa Hospital Research Institute, ²Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, ³Faculty of Medicine, University of Ottawa

This article presents a high-throughput luciferase expression-based method of titrating various RNA and DNA viruses using automated and manual liquid handlers.