Baker IDI Heart and Diabetes Institute
2 ARTICLES PUBLISHED IN JoVE
JoVE Journal
Cholesterol Efflux Assay
Hann Low 1, Anh Hoang 1, Dmitri Sviridov 1
1Baker IDI Heart and Diabetes Institute
The cholesterol assay is designed to quantitate the rate of cholesterol efflux from cultured cells and the capacity of plasma acceptors to accept cholesterol released from cells. The assay consists of labelling cells with cholesterol, equilibration of cholesterol among intracellular pools and release of cholesterol to an extracellular acceptor.
Medicine
Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel
Thomas Bonnard 1, Christoph E. Hagemeyer 1
1Vascular Biotechnology Laboratory, Baker IDI Heart and Diabetes Institute
The FeCl3 induced thrombosis model in mice is described herein. A method to monitor thrombus growth by intravital microscopy observation on a mesenteric vessel and by blood flow measurement in the carotid artery is presented.
