Baker IDI Heart and Diabetes Institute

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

The cholesterol assay is designed to quantitate the rate of cholesterol efflux from cultured cells and the capacity of plasma acceptors to accept cholesterol released from cells. The assay consists of labelling cells with cholesterol, equilibration of cholesterol among intracellular pools and release of cholesterol to an extracellular acceptor.

Medicine

Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel

Thomas Bonnard¹, Christoph E. Hagemeyer¹

¹Vascular Biotechnology Laboratory, Baker IDI Heart and Diabetes Institute

The FeCl₃ induced thrombosis model in mice is described herein. A method to monitor thrombus growth by intravital microscopy observation on a mesenteric vessel and by blood flow measurement in the carotid artery is presented.