Georg August University Medical Center, Göttingen, Germany
2 ARTICLES PUBLISHED IN JoVE
Biology
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
Julia U. Sprenger 1, Ruwan K. Perera 1, Konrad R. Götz 1, Viacheslav O. Nikolaev 1
1Emmy Noether Group of the DFG, Department of Cardiology and Pneumology, European Heart Research Insitute Göttingen, Georg August University Medical Center, Göttingen, Germany
Förster resonance energy transfer (FRET) microscopy is a powerful technique for real-time monitoring of signaling events in live cells using various biosensors as reporters. Here we describe how to build a customized epifluorescence FRET imaging system from commercially available components and how to use it for FRET experiments.
Medicine
Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System
Christiane Jungen 1,2, Katharina Scherschel 1,2, Nadja I. Bork 2,3, Pawel Kuklik 1, Christian Eickholt 1, Helge Kniep 1, Niklas Klatt 1,2, Stephan Willems 1,2, Viacheslav O. Nikolaev 2,3, Christian Meyer 1,2
1Department of Cardiology-Electrophysiology, cNEP (cardiac Neuro- and Electrophysiology research group), University Heart Center, University Hospital Hamburg-Eppendorf, 2DZHK (German Center for Cardiovascular Research), 3Institute of Experimental Cardiovascular Research, University Medical Center Hamburg-Eppendorf
Here, we present a protocol for the modulation of the intracardiac autonomic nervous system and the assessment of its influence on basic electrophysiology, arrhythmogenesis, and cAMP dynamics using an ex vivo Langendorff setup.
