Georg August University Medical Center, Göttingen, Germany

2 ARTICLES PUBLISHED IN JoVE

Biology

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Julia U. Sprenger¹, Ruwan K. Perera¹, Konrad R. Götz¹, Viacheslav O. Nikolaev¹

¹Emmy Noether Group of the DFG, Department of Cardiology and Pneumology, European Heart Research Insitute Göttingen, Georg August University Medical Center, Göttingen, Germany

Förster resonance energy transfer (FRET) microscopy is a powerful technique for real-time monitoring of signaling events in live cells using various biosensors as reporters. Here we describe how to build a customized epifluorescence FRET imaging system from commercially available components and how to use it for FRET experiments.

Medicine

Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System

Christiane Jungen^1,2, Katharina Scherschel^1,2, Nadja I. Bork^2,3, Pawel Kuklik¹, Christian Eickholt¹, Helge Kniep¹, Niklas Klatt^1,2, Stephan Willems^1,2, Viacheslav O. Nikolaev^2,3, Christian Meyer^1,2

¹Department of Cardiology-Electrophysiology, cNEP (cardiac Neuro- and Electrophysiology research group), University Heart Center, University Hospital Hamburg-Eppendorf, ²DZHK (German Center for Cardiovascular Research), ³Institute of Experimental Cardiovascular Research, University Medical Center Hamburg-Eppendorf

Here, we present a protocol for the modulation of the intracardiac autonomic nervous system and the assessment of its influence on basic electrophysiology, arrhythmogenesis, and cAMP dynamics using an ex vivo Langendorff setup.