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Dalton Cardiovascular Research Center

3 ARTICLES PUBLISHED IN JoVE

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Biology

The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation
Pooneh Bagher 1, Steven S. Segal 1,2
1Department of Medical Pharmacology and Physiology, University of Missouri, 2Dalton Cardiovascular Research Center, University of Missouri

A tissue preparation is described for visualization and experimental manipulation of the living microcirculation. In anesthetized male mice, the thin, highly vascularized cremaster muscle is prepared for intravital microscopy to study microvascular networks including arterioles, capillaries and venules. This preparation is readily adapted for rats and hamsters.

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Biology

Microiontophoresis and Micromanipulation for Intravital Fluorescence Imaging of the Microcirculation
Pooneh Bagher 1, Luis Polo-Parada 1,2, Steven S. Segal 1,2
1Department of Medical Pharmacology and Physiology, University of Missouri, 2Dalton Cardiovascular Research Center, University of Missouri

Microiontophoresis entails movement of ions from a micropipette in response to a difference in electrical potential between the inside and outside of the micropipette. Biologically active molecules are thereby delivered in proportion to electrical current. We illustrate acetylcholine microiontophoresis in conjunction with micromanipulation to study endothelium-dependent vasodilation in the microcirculation.

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Biology

Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries
Matthew J. Socha 1, Steven S. Segal 1,2
1Medical Pharmacology and Physiology, University of Missouri, 2Dalton Cardiovascular Research Center

We present a preparation for visualizing and manipulating calcium signaling in native, intact microvascular endothelium. Endothelial tubes freshly isolated from mouse resistance arteries supplying skeletal muscle retain in vivo morphology and dynamic signaling within and between neighboring cells. Endothelial tubes can be prepared from microvessels of other tissues and organs.

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