Research Center Borstel
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei
Ann-Kristin Mueller 1, Jochen Behrends 2, Jannike Blank 2, Ulrich E. Schaible 2, Bianca E. Schneider 2
1Department of Infectious Diseases, Parasitology Unit, University Hospital Heidelberg, 2Priority Area Infections, Research Center Borstel
Tuberculosis and malaria are two of the most prevalent infections in humans and major causes of morbidity and mortality in impoverished populations in the tropics. We established an experimental model system to study outcome of malaria-tuberculosis coinfection in mice after challenge with both pathogens via their natural route of infection.
Immunology and Infection
Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
Kerstin Menck 1, Daniel Behme 1, Mathias Pantke 1, Norbert Reiling 2, Claudia Binder 1, Tobias Pukrop 1, Florian Klemm 1
1Department of Hematology and Oncology, University Medical Center Göttingen, 2Microbial Interface Biology Group, Research Center Borstel
We present a simple and efficient protocol for the generation of human macrophages. Buffy coats are processed by double density gradient centrifugation and isolated monocytes are then differentiated to macrophages in Teflon-coated cell culture bags. This maximizes macrophage yields and facilitates cell harvesting for subsequent experiments.
