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Research Center Borstel

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei

Ann-Kristin Mueller¹, Jochen Behrends², Jannike Blank², Ulrich E. Schaible², Bianca E. Schneider²

¹Department of Infectious Diseases, Parasitology Unit, University Hospital Heidelberg, ²Priority Area Infections, Research Center Borstel

Tuberculosis and malaria are two of the most prevalent infections in humans and major causes of morbidity and mortality in impoverished populations in the tropics. We established an experimental model system to study outcome of malaria-tuberculosis coinfection in mice after challenge with both pathogens via their natural route of infection.

Immunology and Infection

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags

Kerstin Menck¹, Daniel Behme¹, Mathias Pantke¹, Norbert Reiling², Claudia Binder¹, Tobias Pukrop¹, Florian Klemm¹

¹Department of Hematology and Oncology, University Medical Center Göttingen, ²Microbial Interface Biology Group, Research Center Borstel

We present a simple and efficient protocol for the generation of human macrophages. Buffy coats are processed by double density gradient centrifugation and isolated monocytes are then differentiated to macrophages in Teflon-coated cell culture bags. This maximizes macrophage yields and facilitates cell harvesting for subsequent experiments.