University of Delaware
2 ARTICLES PUBLISHED IN JoVE
Biology
High Throughput Microinjections of Sea Urchin Zygotes
Nadezda A. Stepicheva 1, Jia L. Song 1
1Department of Biological Sciences, University of Delaware
Microinjection is a common technique used to deliver DNA constructs, mRNAs, morpholino antisense oligonucleotides or other treatments into eggs, embryos, and cells of various species.
Biology
Primary Cell Cultures from the Mouse Retinal Pigment Epithelium
Peng Shang 1, Nadezda A. Stepicheva 1, Stacey Hose 1, J. Samuel Zigler, Jr. 2, Debasish Sinha 1,2
1Department of Ophthalmology, University of Pittsburgh School of Medicine, 2Wilmer Eye Institute, The Johns Hopkins University School of Medicine
The retinal pigment epithelium (RPE) is a multi-functional epithelium of the eye. Here we present a protocol to establish primary cell cultures derived from the murine RPE.
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