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University of Delaware

2 ARTICLES PUBLISHED IN JoVE

Biology

High Throughput Microinjections of Sea Urchin Zygotes

¹Department of Biological Sciences, University of Delaware

Microinjection is a common technique used to deliver DNA constructs, mRNAs, morpholino antisense oligonucleotides or other treatments into eggs, embryos, and cells of various species.

Biology

Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

Peng Shang¹, Nadezda A. Stepicheva¹, Stacey Hose¹, J. Samuel Zigler, Jr.², Debasish Sinha^1,2

¹Department of Ophthalmology, University of Pittsburgh School of Medicine, ²Wilmer Eye Institute, The Johns Hopkins University School of Medicine

The retinal pigment epithelium (RPE) is a multi-functional epithelium of the eye. Here we present a protocol to establish primary cell cultures derived from the murine RPE.