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2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice
Xi Huang 1, Anuraag Sarangi 2, Tatiana Ketova 1, Ying Litingtung 1, Michael K. Cooper 2, Chin Chiang 1
1Department of Cell and Developmental Biology, Vanderbilt University, 2Department of Neurology, Vanderbilt University
This protocol describes the isolation and dissociation of mouse medulloblastoma tissue, and subsequent allografting of the tumor cells into immunocompromised recipient mice in order to initiate secondary medulloblastoma.
Medicine
Primary Orthotopic Glioma Xenografts Recapitulate Infiltrative Growth and Isocitrate Dehydrogenase I Mutation
J. Geraldo Valadez 1, Anuraag Sarangi 1, Christopher J. Lundberg 1, Michael K. Cooper 1,2,3
1Department of Neurology, Vanderbilt University Medical Center, 2Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, 3Neurology Service, Veteran Affairs TVHS
Malignant gliomas constitute a heterogeneous group of highly infiltrative glial neoplasms with distinct clinical and molecular features. Primary orthotopic xenografts recapitulate the histopathological and molecular features of malignant glioma subtypes in preclinical animal models.
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