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2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice

Xi Huang¹, Anuraag Sarangi², Tatiana Ketova¹, Ying Litingtung¹, Michael K. Cooper², Chin Chiang¹

¹Department of Cell and Developmental Biology, Vanderbilt University, ²Department of Neurology, Vanderbilt University

This protocol describes the isolation and dissociation of mouse medulloblastoma tissue, and subsequent allografting of the tumor cells into immunocompromised recipient mice in order to initiate secondary medulloblastoma.

Medicine

Primary Orthotopic Glioma Xenografts Recapitulate Infiltrative Growth and Isocitrate Dehydrogenase I Mutation

J. Geraldo Valadez¹, Anuraag Sarangi¹, Christopher J. Lundberg¹, Michael K. Cooper^1,2,3

¹Department of Neurology, Vanderbilt University Medical Center, ²Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, ³Neurology Service, Veteran Affairs TVHS

Malignant gliomas constitute a heterogeneous group of highly infiltrative glial neoplasms with distinct clinical and molecular features. Primary orthotopic xenografts recapitulate the histopathological and molecular features of malignant glioma subtypes in preclinical animal models.