Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)

2 ARTICLES PUBLISHED IN JoVE

Biology

Demonstration of Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by Combining Current Measurements with Detection of Cleavage Fragments

Matteus Krappitz¹, Christoph Korbmacher¹, Silke Haerteis¹

¹Institut für Zelluläre und Molekulare Physiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)

Proteolytic activation of the epithelial sodium channel (ENaC) heterologously expressed in Xenopus laevis oocytes can be demonstrated by combining current measurements with a biotinylation approach to investigate the appearance of ion channel cleavage products at the cell surface. Functionally important cleavage sites can be identified by using site-directed mutagenesis.

Education

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions

José R. Agudo¹, Jiwon Han¹, Jinyoung Park¹, Sinyoung Kwon¹, Soebiakto Loekman², Giovanni Luzi¹, Christoph Linderberger³, Antonio Delgado^1,4, Andreas Wierschem⁴

¹Institute of Fluid Mechanics, FAU Busan Campus, University of Erlangen-Nuremberg, ²Institute of Chemical Reaction Engineering, FAU Busan Campus, University of Erlangen-Nuremberg, ³Institute of Bioprocess Engineering, FAU Busan Campus, University of Erlangen-Nuremberg, ⁴Institute of Fluid Mechanics, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)

Two different methods for characterizing the incipient particle motion of a single bead as a function of the sediment bed geometry from laminar to turbulent flow are presented.